Chromogenic TAL Assay (Chromogenic endotoxin test assay)
ʻO TAL reagent ka lyophilized amebocyte lysate i lawe ʻia mai ke koko uliuli o Limulus polyphemus a i ʻole Tachypleus tridentatus.
ʻO nā endotoxins nā lipopolysaccharides amphiphilic (LPS) aia i loko o ka membrane cell waho o nā hua bacteria gram-negative.ʻO nā huahana makua i hoʻohaumia ʻia me nā pyrogens me ka LPS hiki ke alakaʻi i ka ulu ʻana o ke kuni, ka hoʻokomo ʻana i ka pane ʻeha, ka haʻalulu, ka hemahema o ke kino a me ka make i ke kanaka.No laila, ua hoʻokumu nā ʻāina a puni ka honua i nā lula, e koi ana e hoʻāʻo ʻia kekahi huahana lāʻau e ʻōlelo ana he sterile a non-pyrogenic ma mua o ka hoʻokuʻu ʻana.Ua hoʻokumu mua ʻia ʻo Gel-clot TAL assay no ka hoʻāʻo endotoxins bacteria (ie BET).Eia naʻe, ua puka mai nā ʻano hana holomua ʻē aʻe o ka TAL assay.A ʻaʻole e ʻike wale kēia mau ala akā e helu pū i ka hiki ʻana o nā endotoxins i loko o kahi laʻana.
Ma waho aʻe o ka ʻenehana gel-clot, loaʻa nā ʻenehana no ka BET i ka ʻenehana turbidimetric a me ka ʻenehana chromogenic.
ʻO Bioendo, i hoʻolaʻa ʻia i ka ʻike endotoxin, ʻo ia ka mea hana ʻoihana e hoʻomohala maoli i kahi hōʻike chromogenic TAL.BioendoTMHāʻawi ʻo EC Endotoxin Test Kit (End-point Chromogenic Assay) i kahi ana wikiwiki no ka helu endotoxin.Hāʻawi pū mākou iā BioendoTMKC Endotoxin Test Kit (Kinetic Chromogenic Assay) a me ka mea heluhelu microplate incubation ELx808IULALXH, hiki ke hōʻoia i ka hilinaʻi a me ka pono o kāu mau hoʻokolohua.
Ka manawa hoʻouna: Jul-29-2019