Ka hoʻohana ʻana i ka ʻenehana Chromogenic i ka hoʻāʻo ʻana i ka Endotoxins Bacterial

ʻO ka ʻenehana Chromogenic kekahi o nā ʻenehana ʻekolu i loaʻa pū kekahi ʻenehana gel-clot a me ka ʻenehana turbidimetric e ʻike a helu ʻia nā endotoxins mai Gram-negative bacteria me ka hoʻohana ʻana i ka amoebocyte lysate i unuhi ʻia mai ke koko uliuli o ka pāpaʻi lio (Limulus polyphemus a i ʻole Tachypleus tridentatus).Hiki ke hoʻokaʻawale ʻia ma ke ʻano he ho'āʻo endpoint-chromogenic a i ʻole kahi kinetic-chromogenic assay e pili ana i ke kumu hoʻāʻo i hoʻohana ʻia.

ʻO ka loina pane ʻo ia: ʻo ka amebocyte lysate kahi cascade o serine protease enzymes (proenzymes) hiki ke hoʻāla ʻia e nā endotoxins bacteria.Hoʻolaʻa nā Endotoxins i nā proenzymes e hana i nā enzyme i hoʻāla ʻia (kapa ʻia ʻo coagulase), ka mea hope e hoʻokaʻawale i ka māhele o ka substrate kala ʻole, e hoʻokuʻu ana i kahi hua melemele pNA.Hiki ke ana ʻia ka pNA i hoʻokuʻu ʻia ma ka photometrically ma 405nm.A hoʻopili maikaʻi ʻia ka absorbance me ka ʻike endotoxin, a laila hiki ke helu ʻia ka ʻike endotoxin e like me ia.