ʻO ka hoʻopaʻa ʻana i ka Cascade Reagents Chromogenic Assay

ʻO Bioendo rCR Endotoxin Mea hoʻāʻo (ʻO ka hoʻopaʻa ʻana i ka Cascade Reagents Chromogenic Assay) hoʻohana i ka ʻenehana recombinant gene e hōʻike i ka Factor C alahele serine zymogen proteases o nā amebocyte pāpaʻi lio i loko o nā pūnana eukaryotic.Loaʻa i nā cascade reagents ka Factor C i hiki ke hoʻāla ʻia e nā endotoxins bacteria, Factor B a me ka enzyme proclotting.Hoʻopili nā Endotoxins a hoʻololi i ka Factor C i kona ʻano hana, a laila hoʻāla i ka Factor B, a laila hoʻāla i ka enzyme proclotting.Hoʻopau ka enzyme proclotting i hoʻāla ʻia i ka substrate chromogenic, e hoʻokuʻu ana i kahi pNA ʻulaʻula melemele.Hiki ke ana ʻia ka pNA i hoʻokuʻu ʻia ma ka photometrically ma 405 nm.Ma muli o ia mea, hiki ke helu ʻia ka helu endotoxin o ka laʻana hoʻāʻo.ʻAʻole hoʻohana ka pahu hoʻāʻo endotoxin rCR i nā mea kumu holoholona, ​​​​pale i nā pāpaʻi lio.