KCET- Kinetic Chromogenic Endotoxin Test Assay (Chromogenic endotoxin test assay he ala koʻikoʻi no nā laʻana me kekahi keakea.)
ʻO ka ho'āʻo kinetic chromogenic endotoxin (KCT a i ʻole KCET) kahi ala i hoʻohana ʻia e ʻike ai i ka loaʻa ʻana o nā endotoxins i kahi laʻana.
ʻO nā endotoxins nā mea ʻawaʻawa i loaʻa i loko o nā paia cell o kekahi mau ʻano bacteria, me nā hua bacteria gram-negative e like me Escherichia coli a me Salmonella.Ma ka KCET assay, ua hoʻohui ʻia kahi substrate chromogenic i ka laʻana, kahi e hana ai me nā endotoxins i loaʻa e hana i kahi hoʻololi kala.
E nānā ʻia ka nui o ka hoʻomohala ʻana o ka waihoʻoluʻu i ka manawa me ka hoʻohana ʻana i kahi spectrophotometer, a ua helu ʻia ka nui o ka endotoxin i ka hāpana ma muli o kēia helu.
ʻO ka KCT assay kahi ala kaulana no ka ʻike ʻana i nā endotoxins i nā lāʻau lapaʻau, nā mea lapaʻau, a me nā huahana ʻē aʻe e pili ana i ke kino kanaka.He ho'āʻo koʻikoʻi a hilinaʻi e hiki ke ʻike i nā helu liʻiliʻi loa o ka endotoxin, e lilo ia i mea hana koʻikoʻi no ka hōʻoia ʻana i ka palekana o kēia mau huahana.
ʻO TAL/LAL reagent ka lyophilized amebocyte lysate i unuhi ʻia mai ke koko uliuli o Limulus polyphemus a i ʻole Tachypleus tridentatus.
ʻO nā endotoxins nā lipopolysaccharides amphiphilic (LPS) aia i loko o ka membrane cell waho o nā hua bacteria gram-negative.ʻO nā huahana makua i hoʻohaumia ʻia me nā pyrogens me ka LPS hiki ke alakaʻi i ka ulu ʻana o ke kuni, ka hoʻoulu ʻana i ka pane ʻeha, ka haʻalulu, ka hemahema o ke kino a me ka make i ke kanaka.
No laila, ua hoʻokumu nā ʻāina a puni ka honua i nā lula, e koi ana e hoʻāʻo ʻia kekahi huahana lāʻau e ʻōlelo ana he sterile a non-pyrogenic ma mua o ka hoʻokuʻu ʻana.Ua hoʻokumu mua ʻia ʻo Gel-clot TAL assay no ka hoʻāʻo endotoxins bacteria (ie BET).
Eia naʻe, ua puka mai nā ʻano hana holomua ʻē aʻe o ka TAL assay.A ʻaʻole e ʻike wale kēia mau ala akā e helu pū i ka hiki ʻana o nā endotoxins i loko o kahi laʻana.Ma waho aʻe o ka ʻenehana gel-clot, loaʻa nā ʻenehana no ka BET i ka ʻenehana turbidimetric a me ka ʻenehana chromogenic.ʻO Bioendo, i hoʻolaʻa ʻia i ka ʻike endotoxin, ʻo ia ka mea hana ʻoihana e hoʻomohala maoli i kahi chromogenic TAL/LAL assay.
Hāʻawi ʻo Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay) i kahi ana wikiwiki no ka helu endotoxin.
Hāʻawi pū mākou iā Bioendo KC Endotoxin Test Kit (Kinetic Chromogenic Assay) a me ka mea heluhelu microplate incubation ELx808IU-SN, hiki ke hōʻoia i ka hilinaʻi a me ka pono o kāu mau hoʻokolohua.
He aha nā hiʻohiʻona o kakinetic chromogenic endotoxin test assaye ho'āʻo i nā endotoxins i nā laʻana?
ʻO ka kinetic chromogenic endotoxin test assay kekahi ala ʻē aʻe i hoʻohana ʻia e hoʻāʻo ai no nā endotoxins i nā laʻana.Loaʻa iā ia kekahi mau hiʻohiʻona:
1. Ana Kinetic: Like me ka turbidimetric assay, ka kinetic chromogenic assay e pili ana i ke ana kinetic.Ke hilinaʻi nei ia i ka hopena ma waena o nā endotoxins a me kahi substrate chromogenic e hana i kahi huahana kala.Hoʻololi ʻia ka hoʻololi ʻana o ka kala kala i ka manawa, e ʻae ana i ka helu ʻana o nā ʻike endotoxin i ka hāpana.
2. ʻO ke kiʻekiʻe kiʻekiʻe: ʻO ka kinetic chromogenic assay he mea koʻikoʻi loa ia a hiki ke ʻike i nā haʻahaʻa haʻahaʻa o nā endotoxins i nā laʻana.Hiki iā ia ke ana pololei i nā ʻike endotoxin, ʻoiai ma nā pae haʻahaʻa loa, e hōʻoia ana i ka ʻike pono a me ka helu.
3. Ka laulā dynamic ākea: Loaʻa i ka assay kahi ākea ākea ākea, e hiki ai ke ana i nā ʻano endotoxin ma kahi ākea ākea.ʻO ia ke ʻano hiki iā ia ke hoʻāʻo i nā laʻana me nā pae like ʻole o nā endotoxins, e hoʻokipa i nā haʻahaʻa haʻahaʻa a kiʻekiʻe hoʻi me ka ʻole o ka hoʻoheheʻe ʻana a i ʻole ka ʻike.
4. Nā hopena wikiwiki: Hāʻawi ka kinetic chromogenic assay i nā hopena wikiwiki e hoʻohālikelike ʻia me nā ʻano kuʻuna.ʻOi aku ka pōkole o ka manawa hoʻāʻo, hiki i ka hoʻāʻo wikiwiki a me ka nānā ʻana i nā laʻana.Hiki ke nānā ʻia ka hoʻomohala kala i ka manawa maoli, a loaʻa pinepine nā hopena i loko o kekahi mau minuke a i ʻelua mau hola, e pili ana i ka pahu assay kikoʻī a me nā mea hana i hoʻohana ʻia.
5. Automation a standardization: Hiki ke hana ʻia ka assay me ka hoʻohana ʻana i nā ʻōnaehana automated, e like me nā mea heluhelu microplate a i ʻole.
nā mea hoʻopili kikoʻī endotoxin.ʻAe kēia i ka hoʻāʻo kiʻekiʻe-throughput a hōʻoia i nā ana kūlike a me ka maʻamau, e hōʻemi ana i ka hewa kanaka a me ka hoʻonui ʻana i ka pono.
6. Hoʻohālikelike me nā ʻano laʻana like ʻole: Ua kūpono ka kinetic chromogenic assay me kahi ākea o nā ʻano laʻana, me nā lāʻau lapaʻau, nā lāʻau lapaʻau, nā mea olaola, a me nā laʻana wai.He ala maʻalahi ia e hiki ke hoʻohana ʻia i nā ʻoihana like ʻole a me nā noi kahi e koi ʻia ai ka hoʻāʻo endotoxin.
ʻO ka holoʻokoʻa, hāʻawi ka kinetic chromogenic endotoxin test assay i kahi ala maʻalahi, wikiwiki, a hilinaʻi hoʻi no ka ʻike a me ka helu ʻana.
endotoxins i nā laʻana.Hoʻohana nui ʻia ia i ka lāʻau lapaʻau, biotechnology, a me nā ʻoihana mālama olakino no ka mālama maikaʻi a me ka palekana
nā kumu loiloi.
Ka manawa hoʻouna: Jul-29-2019
