ʻO ka hōʻike hoʻāʻo Endotoxin na Lyophilized Amebocyte Lysate (LAL Reagent)

ʻO ka hōʻike hoʻāʻo Endotoxin na Lyophilized Amebocyte Lysate (LAL Reagent)

LAL Reagents: ʻO Lyophilized amebocyte lysate (LAL) he mea hoʻoheheʻe wai o ke koko (amebocytes) mai ka pāpaʻi kāmaʻa ʻAkelanika.
TAL Reagent: ʻO ka TAL reagent he mea hoʻoheheʻe wai o nā keena koko mai Tachypleus tridentatus.
I kēia manawa, ʻo ka hana nui o LAL / TAL reagents aia ma ʻAmelika a me Kina.

ʻO ka hoʻāʻo ʻana o ka gel clot endotoxin test, ʻo ia ke ʻano o nā noi nui ma ka honua holoʻokoʻa, i mea e hoʻokō ai i nā koi palekana o nā lāʻau lapaʻau kanaka.
I kēia manawa, hana ʻo Bioendo a hoʻolako i ka gel clot LAL reagent me nā ampules aniani hōʻike hoʻokahi a me nā hue hoʻāʻo lehulehu.
https://www.bioendo.com/gel-clot-endotoxin-assay/ G01, GS44, G02, G17 a me G52
ʻO kēia ka hopena waiwai no ka ʻike endotoxins qualitative i ka hoʻāʻo lāʻau.ʻOi loa no nā lāʻau injectable a i ʻole nā ​​​​lāʻau makua e hoʻāʻo i nā endotoxins i WFI, API a i ʻole nā ​​​​huahana lāʻau lapaʻau i hoʻopau ʻia.ʻO ka ho'āʻo ho'āʻo Endotoxin kahi koi kiʻekiʻe o nā hana hana, pono ka mea hoʻohana mākaukau e hana i ka gel clot assay e hōʻoia i ka hoʻihoʻi pololei.

He hopena piha noG52ʻO ka hōʻike hoʻāʻo Endotoxin:
LAL Reagent
E hoʻomalu i ka Endotoxin maʻamau
Wai BET
ʻO nā ʻōlelo aʻoaʻo pipette ʻole Endotoxin
ʻO nā paipu aniani ʻole Endotoxin, me ka hana hoʻoheheʻe ʻana a me nā paipu pane.
Incubation mea, e paipai i ka wai auau a maloʻo wela incubator.Pono nā mea hana incubation a pau i ka pololei o ka mahana.
ʻO nā mea hoʻohana a pau e hoʻopā i ka LAL assay e kū ana i ke kūlana o ka pae ʻole endotoxin ” < 0.005EU/ml ”.
Pono ke kaiapuni hoʻokolohua i ka ʻike endotoxin.

E nānā i:
Hoʻohana nui ʻia nā mea hoʻohana palaki e like me ka pipette tips a i ʻole nā ​​​​papa multiwell no ka hoʻāʻo endotoxin.Pono nā Pharmacopoeias (USP/CP) i hoʻohui ʻia i ka ʻole o ka endotoxin hiki ke ʻike ʻia i kēlā me kēia mea i hoʻohana ʻia a me nā paipu aniani.

Pehea e hana ai i ka ho'āʻo endotoxin e ʻike i nā endotoxins i loko o ka laʻana?
ʻO ka mea mua, pono e hoʻokō ʻia ka hoʻāʻo no ka hōʻoia ʻana o ka lysate sensitivity.No ka hoʻopaʻa ʻana i ka naʻau e like me nā kaha lepili.
No ka laʻana, e hana i nā ho'āʻo endotoxin pre-interference.
E hana i ka ho'āʻo endotoxin piha o " ho'āʻo hoʻopilikia ".
E hana i ka ho'āʻo palena no ka loaʻa ʻana o ka pae endotoxins i ka hāpana.

I ka hana ana i ka ho'āʻo no ka hōʻoia 'ana o ka lepili lysate sensitivity, ka hopena he abnormal, 2 Lamada havenot gel hoʻokumu?
E nānā inā pololei ke ʻano o ka hoʻomākaukau ʻana o ka endotoxin standard control bacteria.
Pono ka hui ʻana o Vortex no kēlā me kēia diluent (e ʻike i ka manual aʻoaʻo a i ʻole ka hoʻokomo ʻana o ka endotoxin standard control ma nā kikoʻī).
E nānā inā pili ka lysate reagent i ka endotoxin maʻamau.

Manaʻo ʻia e hoʻohana i kahi ʻauʻau wai a i ʻole kahi incubator wela maloʻo no ka hana wela mau o ka lysate reagent (no ka laʻana, ʻaʻole pono ka incubator a i ʻole kahi pahu maloʻo).
I ka wā o ka incubation, ka wai wai i loko o ka ʻauʻau wai i kūpaʻa a paʻa, pale ʻia nā haʻalulu a pau.
E nānā pono i ka ʻauʻau wai e hoʻopau i ka pā kahe wai.

I ka hoʻoholo ʻana i ka hopena, e lawe mālie i ka pahu hoʻāʻo a i ʻole ka ampoule aniani mai ka thermostat, e hoʻohuli mālie i 180 degere,
ʻAʻole e hoʻopōʻino ʻia ka hoʻokumu ʻana o ka gel i loko o ka paipu, ʻaʻole e paheʻe, ua hoʻopaʻa ʻia ka hopena e ka hōʻailona "+";
ʻaʻole i hoʻokumu ʻia ka gel a ʻaʻole hiki ke mālama ʻia ka gel clot ʻoiai ua hana ʻia.
ʻAʻole maikaʻi ka paheʻe o ka pā, ua hoʻopaʻa ʻia ka hopena e ka hōʻailona "-".
No ka pahu ho'āʻo endotoxin gel clot no ke ʻano hoʻāʻo endotoxin gel clot.
I ka ho'āʻo ʻana o ka endotoxin gel clot wikiwiki, ʻaʻole hoʻopuka ka hoʻokele maikaʻi i ka gel?
ʻO ka mea mua, e hoʻohana i ka wai hoʻāʻo endotoxin bacteria e hōʻoia i ka lysate reagents, e hōʻoia i ka hoʻokō ʻana o nā mea hana a me ke kaiapuni i nā lula;

Inā kūpono ka pahu, a laila, ʻaʻole i hoʻokumu ʻia ka gel hoʻokele maikaʻi o ka hāpana ma muli o ka hopena inhibitory o ka hāpana, a pono e hana ʻia ka hāpana a mālama ʻia.
ʻO ke ʻano hana hoʻohālike maʻamau ka dilution.