ʻO Limulus amebocyte lysate (LAL) a i ʻole Tachypleus tridentatus lysate (TAL) he ʻano wai o nā ʻili koko mai ka pāpaʻi lio.
A ʻo nā endotoxins he mau molekole hydrophobic i ʻāpana o ka lipopolysaccharide complex e hoʻokumu i ka hapa nui o ka membrane waho o ka bacteria Gram-negative.ʻO nā huahana makua i hoʻohaumia ʻia me nā pyrogens hiki ke alakaʻi i nā hopena koʻikoʻi e like me ke kuni, ka haʻalulu, ka hemahema o ke kino, a i ʻole ka make.
Hiki i ka LAL/TAL reagent ke hana me ka endotoxin bacteria a me ka lipopolysaccharide (LPS).ʻO ka hiki ke hoʻopaʻa a me ka hoʻopaʻa ʻana o ka endotoxin o LAL ka mea e waiwai nui ai i kā mākou ʻoihana ponoʻī.A ʻo ia ke kumu e hoʻohana ʻia ai ka reagent LAL/TAL no ka ʻike ʻana a i ʻole ka helu ʻana i ka endotoxin bacteria.
Ma mua o ka ʻike ʻana e hiki ke hoʻohana ʻia ka LAL/TAL no ka hoʻāʻo ʻana i ka endotoxins bacteria, hoʻohana ʻia nā rabbit e ʻike a helu i nā endotoxins i nā huahana lāʻau.Hoʻohālikelike ʻia me RPT, ʻo BET me ka LAL / TAL reagent ka wikiwiki a me ka maikaʻi, a ʻo ia ke ala kaulana e hana i ka nānā ʻana o ka endotoxin i ka ʻoihana lāʻau lapaʻau, a pēlā aku.
ʻO ka gel clot endotoxin test assay, i ʻike ʻia ʻo ka hoʻāʻo Limulus Amebocyte Lysate (LAL), a i kapa ʻia ʻo Lyophilized Amebocyte Lysate (LAL) he ala i hoʻohana nui ʻia no ka ʻike ʻana a me ka helu ʻana i nā endotoxins i nā huahana like ʻole, ʻo ia hoʻi i ka ʻoihana lāʻau lapaʻau a me nā ʻoihana olakino.Ua manaʻo ʻia he hopena kūpono i ke kahua o ka ʻike endotoxin ma muli o kona pono a me ka ʻae ʻana i nā lula.
Hoʻokumu ʻia ka hoʻāʻo LAL ma luna o ka manaʻo e loaʻa i nā pūnaewele koko o nā pāpaʻi lio (Limulus polyphemus a i ʻole Tachypleus tridentatus) kahi mea clotting e hoʻopili me nā endotoxins bacteria, ka hopena i ka hoʻokumu ʻia ʻana o ka gel-like clot.He mea koʻikoʻi loa kēia pane a kikoʻī i nā endotoxins, nā mea ʻawaʻawa o ka membrane waho o ka hua bacteria gram-negative.
Nui nā kumu i manaʻo ʻia ai ka hoʻāʻo ʻana o ka gel clot endotoxin test assay he hopena kūpono i ka ʻike endotoxin:
1. ʻAe Hoʻoponopono: Ua ʻike ʻia ka hoʻāʻo LAL e nā luna hoʻoponopono e like me ka United States Pharmacopeia (USP) a me ka European Pharmacopoeia (EP) ma ke ʻano maʻamau no ka hoʻāʻo endotoxin.Pono ka hoʻokō ʻana i kēia mau lula no ka hōʻoia ʻana i ka palekana a me ka maikaʻi o nā huahana lāʻau.
2. ʻIke a me ke kikoʻī: He kiʻekiʻe ko ka hoʻāʻo LAL, hiki ke ʻike i nā pae haʻahaʻa loa o nā endotoxins.Hiki iā ia ke ʻike i nā ʻāpana endotoxin ma lalo o 0.01 mau ʻāpana endotoxin no ka milliliter (EU/mL).ʻO ka kikoʻī o ka hoʻāʻo e hōʻoia i ka ʻike mua ʻana i nā endotoxins a hōʻemi i nā hopena maikaʻi ʻole.
3. Uku Uku: ʻO ka ho'āʻo ho'āʻo endotoxin gel clot i manaʻo ʻia he hopena hoʻokele waiwai ke hoʻohālikelike ʻia me nā ʻano hana ʻē aʻe e like me ka chromogenic a i ʻole turbidimetric assays.Pono ka liʻiliʻi o nā reagents a me nā mea hana, e hōʻemi ana i nā kumukūʻai hoʻāʻo holoʻokoʻa.Eia kekahi, ʻo ka loaʻa ʻana o nā reagents LAL maʻamau i ka mākeke e maʻalahi i nā laboratories e hana i ka hoʻāʻo.
4. ʻOihana Kūlana: Ua hoʻohana nuiʻia ka ho'āʻo LAL ma nāʻoihana lāʻau lapaʻau a me nā mea lapaʻau e like me keʻano maʻamau no kaʻike endotoxin.He ʻāpana koʻikoʻi ia o nā kaʻina hoʻokele maikaʻi i ka wā o ka hana ʻana i nā huahana lāʻau lapaʻau a me nā mea lapaʻau, e hōʻoia ana i ka hoʻokō ʻana i nā koi hoʻoponopono.
Eia nō naʻe, pono e hoʻomaopopo ʻia he mau palena paha ka gel clot endotoxin test assay, e like me ke keakea ʻana mai kekahi mau mea a me ka hiki ke loaʻa nā hopena maikaʻi ʻole a i ʻole hoʻopunipuni.I nā hihia kikoʻī, hiki ke hoʻohana ʻia nā ala ʻē aʻe e like me ka chromogenic a i ʻole turbidimetric assays e hoʻokō a hōʻoia i nā hopena i loaʻa mai ka hoʻāʻo LAL.
Ka manawa kau: Apr-29-2019
