Hōʻike ʻo Recombinant Factor C (rFC).he ala hoʻohana nui ʻia no ka ʻike ʻana i nā endotoxins bacteria, i ʻike ʻia hoʻi he lipopolysaccharides (LPS), ʻo Endotoxins kekahi ʻāpana o ka membrane waho o Gram-negative bacteria e hiki ai ke hoʻoulu i ka hopena inflammatory ikaika i nā holoholona, me nā kānaka.Hoʻokumu ʻia ka rFC assay ma ka hoʻohana ʻana i kahi ʻano genetically engineered o Factor C, kahi enzyme i loaʻa maoli i loko o ke koko pāpaʻi lio a komo i ke ala hoʻopaʻa.Ma ka rFC assay, hoʻohana ʻia ka Recombinant Factor C e ʻike i ke alo o nā endotoxins ma ke ana ʻana Ma ke ana ʻana i ka ʻike o nā substrates i hoʻokaʻawale ʻia i mua o ka endotoxin.Ke hoʻohālikelike ʻia i nā ʻano hana kuʻuna o ka ʻike endotoxin, e like me ka Limulus Amebocyte Lysate (LAL) assay e hoʻohana ana i ke koko pāpaʻi lio, ua manaʻo ʻia ka rFC assay e ʻoi aku ka maʻamau a me ka reproducible, ʻoiai ʻaʻole ia e hilinaʻi i ka hoʻohana ʻana i nā reagents i loaʻa i nā holoholona.ʻOi aku ka maikaʻi o ke kaiapuni a me ka hoʻomau ʻana o ka rFC assay, ʻoiai e hōʻemi ana i ka pono o ka hōʻiliʻili ʻana a me ka hoʻohana ʻana i nā pāpaʻi lio i ka ʻike endotoxin.
Ua ʻae ʻia ka rFC assay e nā mana hoʻoponopono, e like me United States Pharmacopeia (USP), ka European Pharmacopoeia (EP) a me Chinese Pharmacopoeia (CP) no ka hoʻohana ʻana i ka hoʻāʻo ʻana i ka maikaʻi o nā lāʻau lapaʻau a me nā mea lapaʻau.
ʻO nā pono o ka recombinant factor c assay
Hāʻawi ka Recombinant Factor C (rFC) i nā mea maikaʻi ma mua o nā hana kuʻuna no ka ʻike ʻana i nā endotoxins, e like me ka hoʻāʻo ʻo Limulus Amebocyte Lysate (LAL).ʻO kekahi o nā pōmaikaʻi o ka rFC assay penei:
1. Hoʻohālikelike: ʻO ka rFC assay kahi ʻenehana DNA recombinant e hoʻohana ana i hoʻokahi protein i wehewehe ʻia e like me ka reagent ʻike.ʻO kēia ka mea i ʻoi aku ka maʻamau a me ka liʻiliʻi o ka hoʻololi ʻana i ka hoʻohālikelike ʻana i ka LAL assay, e hilinaʻi nei i ka hoʻohana ʻana i kahi hui paʻakikī o nā protein i unuhi ʻia mai ke koko pāpaʻi lio.
2. Reproducibility: ʻO ka rFC assay he kiʻekiʻe kiʻekiʻe o ka reproducibility, no ka mea, hoʻohana ia i hoʻokahi protein i wehewehe ʻia e like me ka reagent ʻike.Hāʻawi kēia i nā hopena kūlike, ʻoiai ma nā ʻāpana like ʻole a me ka nui o nā reagents.
3. Hoʻemi ʻia ka hoʻohana ʻana i nā holoholona: ʻO ka rFC assay kahi ala ʻoi aku ka maikaʻi o ke kaiapuni a me ke ʻano hoʻomau no ka ʻike ʻana i nā endotoxins, no ka mea, ʻaʻole ia e koi i ka hoʻohana ʻana i nā holoholona ola a i mōhai ʻia, e like me nā pāpaʻi lio.
4. Uku-kumu: 'O ka rFC assay 'oi aku ka maika'i ma mua o ka LAL assay, ma muli o ka emi 'ana o ka pono no nā holoholona ola a me ke 'ano ma'amau o ka assay.
5. Paʻa: Paʻa ka rFC assay a hiki ke hoʻohana ʻia i nā ʻano noiʻi ākea, me ka hoʻāʻo ʻana i ka maikaʻi o nā lāʻau lapaʻau, nā lāʻau lapaʻau, a me nā huahana ʻē aʻe i loaʻa nā endotoxins.
6. ʻApono hoʻoponopono: Ua ʻae ʻia ka rFC assay e nā luna hoʻoponopono e like me United States Pharmacopeia (USP), ka European Pharmacopoeia (EP) a me Chinese Pharmacopoeia (CP) no ka hoʻohana ʻana i ka hoʻāʻo ʻana i ka maikaʻi o nā lāʻau lapaʻau a me nā mea lapaʻau.Hāʻawi kēia i kahi kiʻekiʻe o ka hilinaʻi i ka hilinaʻi a me ka pololei o ka assay.
No ka hoʻokō ʻana i nā ʻano koi like ʻole, hana pū ʻo Bioendo a hāʻawi i ke ʻano kuʻuna o ka gel clot endotoxin test assay kit, wikiwiki gel clot assay kit, quantitative endotoxin test assay kit me ka "kinetic turbidimetric endotoxin test assay kitakinetic chromogenic endotoxin test assay kit” .
Ka manawa hoʻouna: Feb-19-2023
