ʻO nā mea hoʻohana ʻole pyrogen he mau mea hoʻohana me ka ʻole o ka endotoxin exogenous, me nā ʻōlelo aʻoaʻo paipu pyrogen-free (tip box), nā paipu hōʻike pyrogen-free a i ʻole i kapa ʻia ʻo endotoxin free glass tubes, pyrogen-free glass ampoules, endotoxin-free 96-well microplates, a me endotoxin- wai noa (depyrogenated wai hoʻohana i loko o ka bacterial endotoxin ho'āʻo ), endotoxin free buffer a me na mea e ae. a me Kina Pharmaceutica).E pili ana i ka wai maʻemaʻe no ka injection me ka ʻike endotoxin ma lalo o 0.015EU/ml.ʻO kēia ka mana hou loa o Pharmacopoeia, ʻo ka wai BET ka liʻiliʻi ma mua o 0.005EU/ml.ʻO ke kūlana kiʻekiʻe loa ma lalo o 0.001EU / ml hiki ke hana a hoʻolako ʻia e Bioendo.
ʻO nā huaʻōlelo koʻikoʻi e pili ana i nā endotoxins, a me nā endotoxin free consumables, pyrogen a me nā kumu wela ʻelua mau manaʻo like ʻole.Pyrogen: Ua kapa ʻia ʻo pyrogen a i ʻole exothermic factor.nā mea hiki ke hoʻonui i ka wela o ke kino.Puna Wela: He mea e hoopuka ana i ka wela.E like me ke kuni ʻana i nā match, ka lanahu, etc.ʻO nā mea i kapa ʻia ʻo "non-pyrogenic consumables" a me "pyrogenic pane" o kekahi mau mea hana a me nā mea kūʻai aku, he mau inoa hewa ʻole a hoʻopunipuni.Pono nā mea pololei i "Pyrogen Free" a me "Pyrogen Response".
No ke aha e pono ai ka Pyrogen free consumables i ka ho'āʻo hoʻāʻo endotoxin, ʻelua hoʻāʻo hoʻāʻo ʻana o ka gel clot endotoxin a me quantitative endotoxin test assay?
ʻAe, pono nā mea hoʻohana ʻole pyrogen no ka hana ʻana i ka hoʻāʻo endotoxin me ka pololei a me ka hilinaʻi.ʻO ka loaʻa ʻana o nā pyrogens, ʻo ia nā mea hoʻonā wela i loaʻa pinepine ʻia mai nā endotoxins bacteria, hiki ke hoʻopilikia i nā hopena o ka hoʻāʻo a alakaʻi i nā heluhelu wahaheʻe.Hoʻohana ʻia ka hoʻāʻo endotoxin, i ʻike maʻamau ʻia ʻo Limulus amebocyte lysate (LAL) a i kapa ʻia ʻo Lyophilized amebocyte lysate (LAL) test, e ʻike a helu i ka loaʻa ʻana o nā endotoxins bacteria i nā lāʻau lapaʻau, nā mea lapaʻau, a me nā huahana ʻē aʻe.Ke hilinaʻi nei ka ho'āʻo LAL i ka hopena ma waena o ka LAL reagent a me nā endotoxins e hana i kahi pane clotting a chromogenic paha.No ka hōʻoia ʻana i nā hopena pololei, he mea koʻikoʻi ka hoʻohana ʻana i nā mea hoʻopau ʻole mai nā pyrogens.Hiki i nā pyrogens ke hoʻohaumia i nā ʻano mea hoʻokolohua like ʻolenā ipu aniani, nā ʻōlelo aʻoaʻo pipette, nā paipu, a me nā ipu laʻana.Inā hoʻopili nā mea hoʻopau i ka pyrogen me ka LAL reagent a i ʻole nā palapala hoʻāʻo, hiki iā lākou ke hoʻoulu i nā hopena maikaʻi ʻole, e alakaʻi ana i nā manaʻo kuhi hewa e pili ana i ka hele ʻana a i ʻole ka neʻe ʻana o nā endotoxins.Hana ʻia nā mea hoʻohana ʻole Pyrogen a hoʻāʻo ʻia e hōʻemi a hoʻopau paha i ka hele ʻana o nā pyrogens.Hana lākou i nā kaʻina hana hoʻomalu maikaʻi e hōʻoia e hoʻokō lākou i nā kūlana i koi ʻia no ka hoʻāʻo endotoxin.ʻO ka hoʻohana ʻana i kēia mau mea hoʻohana kūikawā e kōkua i ka mālama pono a me ka pololei o ka hoʻāʻo endotoxin test assay, e hōʻoiaʻiʻo ana i nā hopena hilinaʻi a me ka hoʻolaha ʻana i ka palekana o ka maʻi ma nā lāʻau lapaʻau a me nā noi biomedical.
Ka manawa hoʻouna: Nov-07-2022
