ʻO Endotoxins nā molekole lipopolysaccharides hydrophobic liʻiliʻi (LPS) i loaʻa mai i ka bacteria e loaʻa ana i loko o ka membrane o waho o ka bacteria gram-negative.Loaʻa nā Endotoxins i kahi kaulahao polysaccharide kumu, nā kaulahao ʻaoʻao polysaccharide O-specific (O-antigen) a me ka lipid compenent, Lipid A, nona ke kuleana no nā hopena ʻona.Hoʻokahe nui nā maʻi bacteria i ka endotoxin i ka make ʻana o ke kelepona a i ka wā e ulu ikaika ana lākou a puʻunaue.Loaʻa i kahi Escherichia coli hoʻokahi ma kahi o 2 miliona mau molekala LPS ma ke kelepona.
Hiki i ka Endotoxin ke hoʻohaumia i nā labwares, a hiki i kona hele ʻana ke hāʻawi nui i nā hoʻokolohua in vitro a me in vivo.A no nā huahana makua, nā huahana makua i hoʻohaumia ʻia me nā endotoxins me ka LPS hiki ke alakaʻi i ka ulu ʻana o ke kuni, ka hoʻoulu ʻana o ka pane ʻeha, ka haʻalulu, ka hemahema o ke kino a me ka make i ke kanaka.No nā huahana dialysis, hiki ke hoʻololi ʻia ka LPS ma o ka membrane me ka nui o ka pore nui ma o ka kānana hope mai ka wai dialysis a i ke koko, hiki ke hoʻoulu ʻia nā pilikia ʻeha.
ʻIke ʻia ka Endotoxin e ka Lyophilized Amebocyte Lysate (TAL).Ua hoʻolaʻa ʻia ʻo Bioendo no ka noiʻi ʻana, hoʻomohala a hana i ka reagent TAL no nā makahiki he ʻehā.Hoʻopili kā mākou huahana i nā ʻenehana āpau i hoʻohana ʻia e ʻike i ka endotoxin, ʻo ia ka ʻenehana gel-clot, ʻenehana turbidimetric, a me ka ʻenehana chromogenic.
Ka manawa hoʻouna: Jan-29-2019
