EtEraser™ SE Endotoxin Removal Kit
EtEraser™ SE Endotoxin Removal Kit
1. ʻIke huahana
ʻO ka lipopolysaccharide (LPS) he endotoxin bacteria a me ka mea nui o nā paia cell o ka bacteria gramnegative.ʻO ka protein recombinant mai E.coli maʻamau ka pae kiʻekiʻe o nā endotoxins.Pono nui ka wehe ʻana i kēia mau endotoxins no nā kaʻina hana i lalo.
EtEraser SE Endotoxin Removal kitua hoʻolālā ʻia e wehe i ka endotoxin contamination mai nā hoʻonā wai.Hoʻopaʻa ʻia nā kits e hoʻemi i ka pae endotoxin i loko o nā laʻana protein e≥99% ma lalo o 2 mau hola.
Aia i loko o ka pahu kahi kolamu hoʻohemo endotoxin i hoʻopaʻa mua ʻia 1.5 ml, Equilibration Buffer, Regeneration Buffer a me ka pyrogen-freecollection tube a me nā ʻōlelo aʻoaʻo.Loaʻa i ke kolamu ka mana paʻa kiʻekiʻe o> 2,000,000 EU / ml.Hiki ke hoʻohana hou ʻia kēia huahana a hiki i ʻelima mau manawa ke hana hou ʻia.Loaʻa ka resin affinity i ka slurry a hiki ke hana i ka hana biopharmaceutical up-scalingin.
EtEraser SE Endotoxin Removal kitutilizes food grade modifiedε-poly-L-lysineʻO ka ligand affinity kiʻekiʻe no nā endotoxins.ʻO ka resin he mau peʻa agarose paʻa loa a ʻaʻohe hopena cytotoxic i ke kino kanaka.Ma hope o ka hoʻomaʻemaʻe ʻana, ʻaʻohe koena mea ʻawaʻawa i koe i loko o nā laʻana.ʻO kēiapahu hoʻopau endotoxinHiki ke hoʻohana ʻia i nā lāʻau lapaʻau protein, nā lāʻau lapaʻau, nā antibodies, DNA/RNA, polysaccharide a me nā laʻana olaola ʻē aʻe.
2. Nā hiʻohiʻona huahana
• paʻa kiʻekiʻe - ʻaʻole pili i ka hana o ka hapa nui o ka laʻana biological
• ka mana hoʻopaʻa kiʻekiʻe -> 2000000 EU / ml
• ʻona ʻole —–> ʻO nā ligands papa ʻai meaʻai ʻole, ʻaʻohe cytotoxiceffects poly lysine
• ka hoʻihoʻi ʻana i ka protein kiʻekiʻe - >95% ka hoʻihoʻi ʻana i ka protein no nā proteinsamples
• kiʻekiʻe hoʻoneʻe 'oihana — wehe i> 99% endotoxin, endotoxin pae i loko o ka hāpana hiki ke emi ma mua o 0.1 EU/ml ma hope o endotoxinremoving kaʻina.
• ka laulā noi ākea - hiki ke hoʻohana ʻia i ka wehe ʻana i ka endotoxin no nā protein, peptides, antibodies, nā lāʻau lapaʻau, polysaccharide a me nā laʻana olaola ʻē aʻe.