ʻO ka Endpoint Chromogenic Kit EC64405

Hāʻawi ʻo Bioendo EC Endotoxin Test Kits i kahi ʻano ana no ka hopena hoʻāʻo endotoxin quantitative.ʻO ka endotoxin i loko o ka laʻana e hoʻāla i kahi cascade o nā enzymes i loko o Amebocyte Lysate, ʻo ka enzyme i hoʻāla ʻia e hoʻokaʻawale i ka substrate synthetic, e hoʻokuʻu ana i kahi huahana melemele me ka nui o ka absorbance ma 405nm.No ka End-point Chromogenic Endotoxin Test Kit me ka Diazo Coupling, pono ka spectrophotometer maʻamau a i ʻole microplate heluhelu e heluhelu i ka absorbance ma 405nm.ʻO ka spectrophotometer maʻamau me ka hana o ka hoʻokolohua kinetic, ka pololei o ka mana wela a me ka kānana 405nm, a laila e hoʻohana i kēlā spectrophotometer hiki ke heluhelu i ka hopena endotoxin quantitative o ka pahu EC.


Huahana Huahana

ʻO ka pahu hoʻāʻo Chromogenic Endotoxin hōʻike hope (me ka ʻole o Diazo Coupling)

1. ʻIke huahana

Kiko ho'āʻo Chromogenic Endotoxin kiko hope(me ka ʻole o Diazo Coupling) e alakaʻi ʻia e ka hoʻohui ʻana i kahi hopena peptide substrate kala ʻole i loko o ka hui ʻana o Lyophilized Amebocyte Lysate a me ka hoʻāʻo ʻana ma hope o kekahi manawa incubation.Inā loaʻa ka endotoxin i ka laʻana hoʻāʻo, e ulu ana ka waihoʻoluʻu melemele ma ka microplate punawai 96.ʻO kona absorbance (λmax = 405nm) pili i ka endotoxin concentration.Hiki ke helu ʻia ka ʻike ʻana o Endotoxin o ka laʻana hoʻāʻo ma muli o kahi pihi maʻamau.

2. Huahelu huahana

Ka Manaʻo Manaʻo: 0.01-0.1 EU / ml, ka manawa hoʻohālikelike e pili ana i 46 mau minuke

0.1-1 EU / ml, ʻo ka manawa hoʻohālikelike e pili ana i 16 mau minuke.

3. Nā hiʻohiʻona huahana a me ka noi

Hoʻohana ʻia ka End-point Chromogenic Endotoxin Test Kit (me ka ʻole o Diazo Coupling) no ka hoʻohana ʻana i ka ʻike In Vitro a me ka helu o nā endotoxins bacterial gram-negative.Hoʻohui ʻia ka hopena peptide substrate kala ʻole i loko o ka hui ʻana o Lyophilized Amebocyte Lysate a me ka hoʻāʻo ʻana ma hope o kekahi manawa incubation.Hoʻomaka ka kala melemele inā loaʻa ka endotoxin i ka laʻana hoʻāʻo.A laila e hoʻohana i ka spectrophotometer maʻamau a i ʻole ka mea heluhelu pā e heluhelu i ka hoʻopaʻa ʻana ma 405nm.He kūpono ka End-point Chromogenic Endotoxin Test Kit (me ka ʻole o Diazo Coupling) no nā ʻano laʻana biomedical e like me ka protein, kano, plasmid, DNA, RNA endotoxin assay.

Nānā:

ʻO Lyophilized Amebocyte Lysate (LAL) reagent i hana ʻia e Bioendo ua hana ʻia mai ka amebocyte lysate i loaʻa i ke koko o ka pāpaʻi lio.

Heluhelu No.

wehewehe

Kiko kiko

ʻO ka ʻike

(EU/ml)

EC64405

 

Bioendo™ EC Endotoxin Test Kit (End-point Chromogenic Assay),

64 Nā hoʻāʻo / kit

 

2 Lyophilized Amebocyte Lysate, 1.7ml / vial;

2 Wai no BET, 50ml / vial;

2 CSE;

4 Chromogenic Substrate, 1.7ml / vial;

0.1 – 1 EU/ml

EC64405S

0.01 – 0.1 EU/ml;

0.1 – 1 EU/m

Kūlana Huahana:

Ua ʻike ʻia ka naʻau o Lyophilized Amebocyte Lysate a me ka mana o Control Standard Endotoxin e kūʻē i ka USP Reference Standard Endotoxin.Hele mai ka Lyophilized Amebocyte Lysate reagent kits me ke aʻo huahana, Certificate of Analysis.

 

Pono anei ka pahu ho'āʻo endotoxin hope loa i ka mea heluhelu microplate akamai?

ʻO Bioendo EC64405 a me EC64405S kit kānana i nā hopena endotoxin quantitative ma o ka heluhelu microplate maʻamau.

Ka helu hope chromogenic endotoxin moʻo pahu hōʻike:

Endotoxin Free Tube

Nā ʻōlelo aʻoaʻo manuahi Pyrogen

Pyrogen Free Microplates

Heluhelu Microplate mau

Endotoxin maʻamau (CSE10V)

Hoʻohana ʻia ka wai BET no ka hoʻoponopono hou ʻana.

 



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