ʻO ka Endpoint Chromogenic Kit EC80545
ʻO Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)
1. ʻIke huahana
Hāʻawi ʻo Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) i kahi ana wikiwiki no ka helu endotoxin.ʻO ka endotoxin i loko o ka laʻana e hoʻāla i kahi cascade o nā enzymes i loko o Amebocyte Lysate, ʻo ka enzyme i hoʻāla ʻia e hoʻokaʻawale i ka substrate synthetic, e hoʻokuʻu ana i kahi mea melemele.A laila hiki i ka mea melemele ke hana hou aku me ka diazo reagents e hana i nā mea poni me ka nui o ka absorbance ma 545nm.Pono ʻia kahi spectrophotometer maʻamau a i ʻole microplate heluhelu no ka hoʻāʻo.Hoʻohālikelike ʻia nā mea ʻulaʻula me ka ʻike endotoxin.A laila ʻo ka hopena o ka hoʻāʻo endotoxin ʻo ka quantitative analysis.
2. Lahui Huahana
Māhele Hoʻonaʻauao: 0.01-0.1EU/ml (manawa hoʻāʻo ma kahi o 46 mau minuke)
0.1-1.0EU/ml (manawa ho'āʻo ma kahi o 16 mau minuke)
3. Nā hiʻohiʻona huahana a me ka noi
Hoʻohana ʻia ʻo Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) no ka hoʻohana ʻana i ka ʻike In Vitro a me ka helu ʻana o nā endotoxins bacterial gram-negative.Hoʻohui ʻia ka solution substrate peptide artificial kala ʻole i loko o Lyophilized Amebocyte Lysate, a laila e hoʻāʻo i ka hui ʻana.Inā loaʻa ka endotoxin i loko o ke ʻano, hoʻololi ke kala o ka hui ʻana.Ua pili ka absorbance i ka endotoxin concentration.No laila hiki ke hoʻopaʻa ʻia nā pae endotoxn i loko o ka hui ʻana o nā specimen e kūʻē i kahi pihi maʻamau.Ua lawa ka spectrophotometer maʻamau me 540 – 545nm kānana e helu i ka endotoxin me kā mākou EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).
Nānā:
ʻO Lyophilized Amebocyte Lysate (LAL) reagent i hana ʻia e Bioendo ua hana ʻia mai ka amebocyte lysate i loaʻa i ke koko o ka pāpaʻi lio.
| Heluhelu No. | wehewehe | Kiko kiko | Hoʻonaʻauao EU/ml |
| EC80545 | ʻO ka pahu hoʻāʻo ʻo Bioendo™ EC Endotoxin (Hōʻuluʻulu Chromogenic Assay, Diazo Coupling), 80 Ho'āʻo / Kit | 5 Lyophilized Amebocyte Lysate, 1.7ml / vial; 4Wai no BET, 50ml/boule; 5 CSE; 5 Chromogenic Substrate, 1.7ml / vial; 5 Diazo Reagent 1, 10ml / vial; 5 Diazo Reagent 2, 10ml / vial; 5 Diazo Reagent 3, 10ml / vial; | 0.1 – 1 EU/ml |
| EC80545S | 0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
Ua ʻike ʻia ka naʻau o Lyophilized Amebocyte Lysate a me ka mana o Control Standard Endotoxin e kūʻē i ka USP Reference Standard Endotoxin.Hele mai ka Lyophilized Amebocyte Lysate reagent kits me ke aʻo huahana, Certificate of Analysis.
Pono anei ka pahu ho'āʻo endotoxin hope loa i ka mea heluhelu microplate akamai?
ʻO Bioendo EC80545 a me EC80545S, hiki ke heluhelu me ka spectrophotometer maʻamau.
Waiho i kāu mau memo
Nā huahana pili
-
ʻO ka Endpoint Chromogenic Kit EC64405
End-point Chromogenic Endotoxin Test Kit (me ka ʻole o ka Diazo Coupling) 1. ʻIke ʻia nā huahana End-point Chromogenic Endotoxin Test Kit (me ka ʻole o Diazo Coupling) e alakaʻi ʻia ma ka hoʻohui ʻana i kahi solution substrate peptide artificial kala ʻole i loko o ka hui ʻana o Lyophilized Amebocyte Lysate a me ka laʻana hoʻāʻo ma hope o kekahi. wā incubation.Inā loaʻa ka endotoxin i ka laʻana hoʻāʻo, e ulu ana ka waihoʻoluʻu melemele ma ka microplate punawai 96.ʻO kona absorbance (λmax = 405nm) pili i ka endotoxin concentration.Endotoxin ...
