Ke alakaʻi kūʻai o Bioendo End-point Chromogenic LAL Test Assay Kit

alakaino ka Bioendo End-point Chromogenic LAL Test Assay Kits:

Hoʻohana mau ʻia ka TAL reagent, ʻo ia hoʻi ka lyophilized amebocyte lysate i unuhi ʻia mai ke koko uliuli o ka pāpaʻi kahakai (Limulus polyphemus a i ʻole Tachypleus tridentatus), e hana i ka hoʻāʻo endotoxins bacteria.

Ma Bioendo, hana mākou i nā kits e hana i ka hōʻike chromogenic TAL assay / LAL assay, kahi e komo ai nā reagents a pau e pono ai no BET ma o ka hoʻololi ʻana i ka hoʻomohala kala, a ʻae i ka mea kūʻai aku e loaʻa nā hopena pololei i kahi manawa pōkole.

ʻO nā loina o ka ʻike chromogenic kiko hope me ka TAL reagent ʻo ia: hiki mai ka hopena enzymatic i ka wā e hoʻāla ai nā endotoxins bacteria i ka mea C, a hoʻokumu ʻia ka coagulase e like me ia.A laila, hoʻokaʻawale ka coagulase i ke pani waihoʻoluʻu ʻole e hoʻokuʻu i kahi pNA hua melemele.Hiki ke ana ʻia ka pNA i hoʻokuʻu ʻia ma ka photometrically ma 405nm.Ma muli o ka hoʻopili maikaʻi ʻana o ka absorbance me ka ʻike endotoxin, hiki ke helu ʻia ka ʻike endotoxin e like me ia.

ʻO EC64405, ʻo ia hoʻi ʻo BioendoEC Endotoxin Test Kit (End-point Chromogenic Assay), kahi pahu hoʻāʻo Chromogenic TAL / LAL assay kit hiki ke hoʻohana ʻia e hana i ka ʻike nui o ka endotoxin bacteria i loko o kahi laʻana.ʻO ka pahu EC64405 i hana ʻia e Bioendo e ʻae i nā hoʻokolohua 64, a ʻo ka naʻau mai ka 0.1 EU / ml a i ka 1 EU / ml.Aia i loko o ka Bioendo EC64405 nā reagents a pau e pono ai no ka ho'āʻo chromogenic hope.No ka mea, loaʻa iā mākou nā mea endotoxin-free a i ʻole pyrogen-free, hiki iā mākou ke hoʻopaʻa i ka pahu e pili ana i kāu mea e pono ai.

Pono ka Incubating Microplate Reader no ka ho'āʻo chromogenic hope.ʻO kā mākou mea heluhelu microplate incubating ELx808IULALXH (ke kumu hoʻohālike hou ma lalo o ka hana ʻana a e hoʻomaka koke ʻia) hiki ke ʻike ʻia nā laʻana like ʻole i loko o ka microplate 96-well i ka manawa like a e kālailai i ka ʻike endotoxin me ka pololei a pololei.

ʻO ka hoʻāʻo hoʻāʻo LAL chromogenic hope me ʻelua pūʻulu kit, hoʻohana kekahi i ka microplate heluhelu e heluhelu i ka hopena e ka pyrogen-free microplate, ʻo kekahi e hoʻohana i ka spectrophotometer maʻamau e heluhelu i ka hopena e nā paipu aniani ʻole endotoxin.

ʻO Lyophilized Amebocyte Lysate / LAL Reagent i hana ʻia e Bioendo i hana ʻia mai ka amebocyte lysate i loaʻa i ke koko o ka pāpaʻi lio.