He kuleana koʻikoʻi ka wai ʻole Endotoxin i ka pololei a me ka hilinaʻi o ka hana hoʻāʻo endotoxin.ʻO nā Endotoxins, i ʻike ʻia hoʻi he lipopolysaccharides (LPS), he mau mea ʻawaʻawa i loaʻa i loko o nā paia cell o Gram-negative bacteria.Hiki i kēia mau mea haumia ke hōʻeha nui i nā kānaka a me nā holoholona inā ʻaʻole i wehe ʻia mai nā huahana lapaʻau e like me nā lāʻau lapaʻau, nā lāʻau lapaʻau, a me nā mea lapaʻau.
No ka ʻike ʻana a me ka helu pono ʻana i nā pae endotoxin, hilinaʻi ka hoʻāʻo endotoxin i kahi hoʻokolohua koʻikoʻi e pono ai ka hoʻohana ʻana i ka wai ʻole endotoxin.Hana ʻia kēia ʻano wai no ka wehe ʻana i nā ʻāpana āpau o nā endotoxins, me ka hōʻoia ʻana i nā hopena maikaʻi i hana ʻia e ka hoʻāʻo ʻana ma muli wale nō o ka loaʻa ʻana o nā endotoxins i loko o ka hāpana e hoʻāʻo ʻia, ʻaʻole ka hopena o ka haumia mai ka wai.
ʻO ka hoʻohana ʻana i ka wai endotoxin ʻole e kōkua i ka hōʻemi ʻana i nā hopena maikaʻi ʻole, hiki ke loaʻa ke loaʻa ka nui o nā endotoxins i loko o ka wai i hoʻohana ʻia i ka hoʻāʻo.Hiki i kēia ke alakaʻi i nā hopena pololei, hiki ke hoʻopaneʻe i ka hoʻokuʻu ʻana i nā huahana a me nā pilikia hoʻoponopono.
I ka hōʻuluʻulu ʻana, he mea koʻikoʻi ka wai endotoxin-free o ka hana hoʻāʻo hoʻāʻo endotoxin, e hōʻoia ana i ka pololei a me ka hilinaʻi o kēia hoʻokolohua koʻikoʻi.Ma ka hōʻemi ʻana i ka hopena o nā hopena maikaʻi ʻole a me ka hōʻoia ʻana e hoʻopuka ʻia nā hopena maikaʻi ma ke alo o ka contamination endotoxin maoli, hana nui ka wai endotoxin-free i ka hōʻoia ʻana i ka palekana a me ka maikaʻi o nā huahana lapaʻau no ka hoʻohana ʻana i nā maʻi.
ʻO ka wai hoʻāʻo endotoxin bacteria
ʻO ka ʻokoʻa ma waena o ka wai hoʻāʻo endotoxin bacteria a me ka wai maʻemaʻe no ka injection: pH, endotoxin bacterial a me nā mea hoʻopilikia.
ʻO ka wai hoʻāʻo endotoxin bacteria
ʻO ka ʻokoʻa ma waena o ka wai hoʻāʻo endotoxin bacteria a me ka wai maʻemaʻe no ka injection: pH, endotoxin bacterial a me nā mea hoʻopilikia.
1. pH
ʻO ka pH kūpono loa no ka hopena ma waenaLAL reagenta ʻo ka endotoxin ka 6.5-8.0.No laila, i ka ho'āʻo LAL, ʻo ʻAmelika Hui Pū ʻIa, ka Japanese Pharmacopoeia a me ka 2015 edition o ka Chinese Pharmacopoeia e kuhikuhi ana i ka waiwai pH o ka huahana hoʻāʻo e hoʻoponopono ʻia i 6.0-8.0.ʻO ka waiwai pH o ka wai no ka hoʻāʻo ʻana i ka endotoxin bacteria e mālama mau ʻia ma 5.0-7.0;ʻO ka pH waiwai o ka wai sterile no ka injection pono e hoʻomaluʻia ma 5.0-7.0.No ka mea he waika nawaliwali ka nui o na laau lapaau, ua maikai ka pH waiwai o ka wai no ka ho'āʻo endotoxin bacteria no ka ho'āʻo endotoxin a i ʻole Lyophilized amebocyte lysate test assay.
2. ʻO ka Endotoxin Bacterial
Pono ka nui o ka endotoxin i loko o ka wai no ka ho'āʻo endotoxin bacteria ma lalo o 0.015EU no 1ml, a ʻo ka nui o ka endotoxin i loko o ka wai no ka hoʻāʻo ʻana i ka endotoxin bacteria ma nā ʻano quantitative e emi iho ma mua o 0.005EU no 1ml;Pono e loaʻa ka wai maʻemaʻe no ka inikua ma lalo o 0.25 EU o endotoxin no 1ml.
Pono ka endotoxin i loko o ka wai no ka ho'āʻo endotoxin bacteria i mea haʻahaʻa i ʻole e hoʻopilikia i nā hopena hōʻike.Inā hoʻohana ʻia ka wai maʻemaʻe no ka inikini ma kahi o ka wai hoʻāʻo no ka hoʻāʻo Endotoxin, ma muli o ke kiʻekiʻe o ka endotoxin maʻiʻo i loko o ka wai sterile no ka injection, ka wai sterile no ka injection a ʻO ka superposition o ka endotoxin i ka laʻana i hoʻāʻo ʻia e hoʻopuka i nā hopena maikaʻi ʻole, e alakaʻi ana i nā poho waiwai. i ka ʻoihana.Ma muli o ka ʻokoʻa o ka maʻiʻo endotoxin, ʻaʻole hiki ke hoʻohana i ka wai sterile no ka inikia ma kahi o ka wai nānā no ka endotoxin test assay a i ʻole Lyophilized amebocyte lysate test assay.
3. Nā Kumu Hoʻopilikia
ʻAʻole pono ka wai no ka hoʻāʻo endotoxin bacteria e hoʻopilikia i ka LAL reagent, e hoʻomalu i ka endotoxin maʻamau a me ka hoʻāʻo LAL;ʻaʻohe koi no ka wai sterile no ka injection.Pono ka wai maʻemaʻe no ka hoʻokō ʻana i ka palekana a me ka paʻa, akā e pili ana ka wai sterile no ka injection i ka hana a me ka paʻa o ka endotoxin standard control bacterial?E hoʻonui a hoʻopaʻa paha i ka hoʻāʻo endotoxin i ka wai maʻemaʻe no ka injection?Kakaʻikahi ka poʻe i hana i ka noiʻi lōʻihi e pili ana i kēia.Ua hōʻoia ʻia ma o ka hoʻokolokolo ʻana ua loaʻa i kekahi wai maʻemaʻe no ka hoʻoheheʻe ʻana i ka hopena pale ikaika i ka hoʻāʻo LAL.Inā hoʻohana ʻia ka wai maʻemaʻe no ka inikia ma kahi o ka wai hoʻāʻo no ka hoʻāʻo ʻana i ka LAL, hiki mai nā hewa hewa ʻole, ka hopena i ka ʻike ʻole ʻia o ka endotoxin, e hoʻoweliweli pololei ana i ka palekana o ka lāʻau.Ma muli o ka loaʻa ʻana o nā kumu hoʻopilikia o ka wai sterile no ka hoʻoinu ʻana, ʻaʻole hiki ke hoʻohana i ka wai sterile no ka injection ma kahi o ka wai nānā no ka hoʻāʻo LAL.
Inā hiki ke hōʻoia ʻia ka pololei o ka wai holoi, ke ʻano holoi a me ka wai hoʻāʻo, ʻaʻole hiki ke hoʻokumu ʻia ka mana maikaʻi i ka hoʻāʻo Limulus, ke ʻole ka maʻamau i hoʻohana ʻia.I mea e hōʻoia ai i ka pololei o nā hopena hōʻike, pono mākou:
a.Kamaʻāina me nā kūlana a me nā kūlana ʻoihana;
b.E hoʻohana i nā huahana kūpono a me nā huahana maʻamau;
c.Hana e like me nā kaʻina hana.
Ka manawa hoʻouna: Iulai-26-2023
